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The transfection protocol was consistent with previous reports ( 2.13 Western blot Proteins were extracted from colon tissue and cell samples and stored at 20C
Treatment with L-Carnitine (300 mg/kg) and alpha-lipoic acid (100 mg/kg) for 28-30 days in aged rats restores activities of succinate dehydrogenase, malate dehydrogenase, -ketoglutarate dehydrogenase, isocitrate dehydrogenase, and respiratory complexes I-IV to near-normal levels, indicating enhanced mitochondrial energy-producing capacity
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